Reproductive Biology and Endocrinology volume 18 , Article number: 17 Cite this article. Metrics details. Sperm cryopreservation has been widely used in assisted reproductive technology ART and has resulted in millions of live births. Two principal approaches have been adopted: conventional slow freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers.
Human sperm vitrification: the state of the art
Sperm Vitrification | SpringerLink
Does cryoprotection of spermatozoa using a vitrification protocol with improved cryoprotective agents and a novel device for large storage lead to better outcomes than conventional slow freezing? Vitrification of human sperm using sucrose and dextran-based cryoprotectant CPA4 with a new vitrification device resulted in significantly better sperm motility and progressive motility and improved DNA integrity with lower DNA fragmentation compared with conventional slow freezing. A major limitation to clinical implementation of vitrification is the right balance between the volume of spermatozoa suspension cryopreserved and a standardised use of CPAs for survival of spermatozoa. This was a control versus current clinical practice study using 30 fresh human semen samples to carry out the different cryoprotectant analyses followed by a further 23 semen samples to test the novel vitrification protocol. Among the 6 cryopreservation methods in this study, vitrification with the funnel-shaped device using CPA4 best preserves the 13 sperm parameters evaluated by CASA system. Conventional slow freezing and vitrification with the device using seminal plasma also protects sperm quality, but the overall motilities are statistically lower in comparison with the novel vitrification approach with cryoprotective media using the device. Extensive training is required to minimise the human error in using the vitrification device to perform cryopreservation.
Cryopreservation of Human Spermatozoa: A New Frontier in Reproductive Medicine
Vitrification in Assisted Reproduction pp Cite as. Sperm cryopreservation has immense potential and serves as a powerful tool in the field of assisted reproductive technology. Slow-freezing protocol has been routinely used for cryopreservation of human spermatozoa.
Cryopreservation is a worldwide technique that makes it possible to preserve different living cells and tissues, including male and female gametes and embryos, in a structurally intact state using low temperature over time. Since the starting point of the cryopreservation era in , until today, this was one of the most important steps in assisted reproductive techniques. Conventional slow freezing of spermatozoa is commonly used for cryopreservation of both ejaculated and surgically retrieved spermatozoa. The technique of the slow freezing is principally based on dehydration of cells which is performed through slow cooling combined with low concentrations of a cryoprotectant agent for achieving a balance. Besides of slow freezing, for more than a decade, many reports suggest the sperm vitrification technique as an alternative to slow freezing.